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human gingival fibroblasts hgfs  (ATCC)


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    Structured Review

    ATCC human gingival fibroblasts hgfs
    CCK-8 value of <t>HGFs</t> of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival <t>fibroblasts;</t> PBS, phosphate-buffered saline (control), ns, P > .05).
    Human Gingival Fibroblasts Hgfs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+gingival+fibroblast+hgf+1/HGF-1/pmc12993141-47-24-29
    Average 96 stars, based on 1520 article reviews
    human gingival fibroblasts hgfs - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis"

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis

    Journal: International Dental Journal

    doi: 10.1016/j.identj.2026.109481

    CCK-8 value of HGFs of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival fibroblasts; PBS, phosphate-buffered saline (control), ns, P > .05).
    Figure Legend Snippet: CCK-8 value of HGFs of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival fibroblasts; PBS, phosphate-buffered saline (control), ns, P > .05).

    Techniques Used: CCK-8 Assay, Cell Counting, Saline, Control

    Cytocompatibility of HGFs treated with Ag-Est816 nanocomposite and PBS (control) at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; PBS, Phosphate-buffered Saline (Control). Immunofluorescence staining: F‑actin labelled the cytoskeleton in red, DAPI labelled nuclei in blue.
    Figure Legend Snippet: Cytocompatibility of HGFs treated with Ag-Est816 nanocomposite and PBS (control) at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; PBS, Phosphate-buffered Saline (Control). Immunofluorescence staining: F‑actin labelled the cytoskeleton in red, DAPI labelled nuclei in blue.

    Techniques Used: Control, Saline, Immunofluorescence, Staining

    Related Articles

    Modification:

    Article Title: Magnolia kobus Extract Suppresses Porphyromonas gingivalis LPS-Induced Proinflammatory Cytokine and MMP Expression in HGF-1 Cells and Regulates Osteoclastogenesis in RANKL-Stimulated RAW264.7 Cells
    Article Snippet: .. The human gingival fibroblast (HGF-1) and murine macrophage RAW264.7 cell lines (American Type Culture Collection, Rockville, MD, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) in a humidified incubator with 5% CO 2 at 37 °C. ..

    Article Title: Effects of Monolaurin on Oral Microbe–Host Transcriptome and Metabolome
    Article Snippet: .. Human gingival fibroblast – HGF-1 (ATCC ® CRL-2014) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Lonza, Walkersville, MD, United States) with 10% Fetal Bovine Serum (FBS) (Lonza, Walkersville, MD, United States). .. Human gingival epithelial cells (keratinocyte OBA-9) were cultured in specific medium for keratinocytes, Defined Keratinocyte-SFM (Life Technologies, Carlsbad, CA, United States) ( ; ).

    Article Title: Magnolia kobus Extract Suppresses Porphyromonas gingivalis LPS-Induced Proinflammatory Cytokine and MMP Expression in HGF-1 Cells and Regulates Osteoclastogenesis in RANKL-Stimulated RAW264.7 Cells.
    Article Snippet: .. The human gingival fibroblast (HGF-1) and murine macrophage RAW264.7 cell lines (American Type Culture Collection, Rockville, MD, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) in a humidified incubator with 5% CO2 at 37 ◦C. ..

    Cell Culture:

    Article Title: Effects of Monolaurin on Oral Microbe–Host Transcriptome and Metabolome
    Article Snippet: .. Human gingival fibroblast – HGF-1 (ATCC ® CRL-2014) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Lonza, Walkersville, MD, United States) with 10% Fetal Bovine Serum (FBS) (Lonza, Walkersville, MD, United States). .. Human gingival epithelial cells (keratinocyte OBA-9) were cultured in specific medium for keratinocytes, Defined Keratinocyte-SFM (Life Technologies, Carlsbad, CA, United States) ( ; ).



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    CCK-8 value of <t>HGFs</t> of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival <t>fibroblasts;</t> PBS, phosphate-buffered saline (control), ns, P > .05).
    Human Gingival Fibroblasts Hgfs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts <t>(HGF-1)</t> labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.
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    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts <t>(HGF-1)</t> labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.
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    human gingival fibroblast cells hgf 1  (ATCC)
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    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts <t>(HGF-1)</t> labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.
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    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts <t>(HGF-1)</t> labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.
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    Image Search Results


    CCK-8 value of HGFs of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival fibroblasts; PBS, phosphate-buffered saline (control), ns, P > .05).

    Journal: International Dental Journal

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis

    doi: 10.1016/j.identj.2026.109481

    Figure Lengend Snippet: CCK-8 value of HGFs of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival fibroblasts; PBS, phosphate-buffered saline (control), ns, P > .05).

    Article Snippet: The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis., , Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments.

    Techniques: CCK-8 Assay, Cell Counting, Saline, Control

    Cytocompatibility of HGFs treated with Ag-Est816 nanocomposite and PBS (control) at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; PBS, Phosphate-buffered Saline (Control). Immunofluorescence staining: F‑actin labelled the cytoskeleton in red, DAPI labelled nuclei in blue.

    Journal: International Dental Journal

    Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis

    doi: 10.1016/j.identj.2026.109481

    Figure Lengend Snippet: Cytocompatibility of HGFs treated with Ag-Est816 nanocomposite and PBS (control) at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; PBS, Phosphate-buffered Saline (Control). Immunofluorescence staining: F‑actin labelled the cytoskeleton in red, DAPI labelled nuclei in blue.

    Article Snippet: The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis., , Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments.

    Techniques: Control, Saline, Immunofluorescence, Staining

    (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts (HGF-1) labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.

    Journal: RSC Advances

    Article Title: Genipin-crosslinked CeO 2 -incorporating Janus patch for oral ulcer treatment

    doi: 10.1039/d6ra00275g

    Figure Lengend Snippet: (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts (HGF-1) labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.

    Article Snippet: Human gingival fibroblasts (HGF-1) were purchased from ATCC (Manassas, USA).

    Techniques: Comparison, Fluorescence, Labeling, Co-Culture Assay, CCK-8 Assay, Staining, Isolation